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. 2022 Feb 8;13(2):121. doi: 10.1038/s41419-022-04588-0

Fig. 3. MYOCD activated the transcription of miR-30a by binding to the CArG box.

Fig. 3

The level of miR-30a-5p was examined in MYOCD overexpression (A) or knockdown (B) cells by RT-PCR (*P < 0.05, **P < 0.01, n = 3). C A schematic diagram of the miR-30a-5p promoter region represents wild-type and mutant CArG boxes. D Luciferase reporter plasmids together with pcDNA3.1-MYOCD plasmids or vectors were cotransfected into HA-VSMCs, and 24 h after transfection, luciferase activity was assayed (**P < 0.01, n = 3). E HA-VSMCs were transfected with luciferase reporter plasmids together with shControl or shMYOCD plasmids for 24 h, and then luciferase activity was assayed (**P < 0.01, n = 3). F HA-VSMCs were transfected with luciferase reporter plasmids in the presence or absence of PDGF-BB for 24 h, and then luciferase activity was assayed (**P < 0.01, n = 3). G 48 h after transfection of pcDNA3.1-MYOCD-myc vector expression plasmids or vector plasmids, ChIP assays were performed using antibodies against myc (**P < 0.01, n = 3). H MiR-30a-5p mimics were transfected into MYOCD knockdown cells, and then western blotting was performed (*P < 0.05, **P < 0.01, n = 3).