Fig. 2.

Chromatin disassembly and reassembly during homologous recombination. After DSB induction, a single nucleosome at the DSB is disassembled and the two adjacent nucleosomes reposition themselves away from the DSB, prior to DNA end resection. Subsequently, extensive chromatin disassembly around the DSB occurs concomitant with DNA end resection during HR, and the removed histones are degraded. Chromatin also appears to be reassembled during HR to promote various steps during HR, and once HR is complete chromatin is reestablished fully and the cell cycle checkpoint is inactivated via checkpoint recovery. The question marks indicate likely events, but that have not yet been proven to occur. The font color corresponds to either yeast or mammalian systems, as indicated in the key.