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. Author manuscript; available in PMC: 2022 Mar 24.
Published in final edited form as: Science. 2021 Aug 26;373(6562):1532–1536. doi: 10.1126/science.abf7461

Fig. 1. A reporter for retinal binding proteins predicts sites of lateral root organogenesis in Arabidopsis.

Fig. 1.

(A) The chemical structure and fluorescence properties of merocyanine aldehyde (MCA) in the absence and presence of a lysine residue from a retinal binding protein. (B) Time course of MCA fluorescence dynamics in Arabidopsis roots. * and ** indicate the site of fluorescent oscillations in the early differentiation zone of the root. (C) Representative images showing the spatial and temporal dynamics of MCA oscillations and the root clock (pDR5:LUC) in a growing root. (D) The pulse of MCA fluorescence indicates the presumptive pre-branch site (PBS) before pDR5:LUC (time is measured from the start of the pDR5:LUC root clock oscillation). (E) A representative example of the temporal signal from MCA and pDR5:LUC in the region of the root that becomes the PBS (this graph was derived from the root shown in panel C).