Table 1.
The result of NGS and Sanger sequencing for TERT promoter region
| No | Tumor | DNA concentration (ng/μl) | NGS data | Sanger sequencing | |||||
|---|---|---|---|---|---|---|---|---|---|
| TMB | MSI | NGS | TERT VAF (%) | TERT TD | TERT TV (%) | ||||
| 1 | Liver | 20 | TMB-low | MSS | C228T | 45.6 | 456 | 80 | C228T |
| 2 | Liver | 14 | TMB-low | MSS | C228T | 25.9 | 201 | 80 | C228T |
| 3 | Liver | 9 | TMB-low | MSS | C228T | 25.6 | 78 | 70 | C228T |
| 4 | Liver | 31 | TMB-high | MSS | C228T | 15.4 | 259 | 20 | C228T |
| 5 | Liver | 22 | TMB-low | MSS | C228T | 36.0 | 114 | 90 | C228T |
| 6 | Liver | 27 | TMB-low | MSS | C228T | 41.7 | 211 | 80 | C228T |
| 7 | Liver | 9 | TMB-low | MSS | C228T | 7.7 | 78 | 60 | C228T |
| 8 | Liver | 33 | TMB-high | MSS | C228T | 44.7 | 94 | 40 | C228T |
| 9 | Liver | 6.4 | TMB-low | MSS | C228T | 26.8 | 112 | 60 | C228T |
| 10 | Liver | 16 | TMB-low | MSS | C228T | 34.1 | 552 | 60 | C228T |
| 11 | Liver | 11 | TMB-low | MSS | C228T | 24.7 | 178 | 70 | C228T |
| 12 | Liver | 47 | TMB-low | MSS | C228T | 28.8 | 66 | 20 | C228T |
| 13 | Liver | 26 | TMB-low | MSS | C250T | 27.9 | 1223 | 70 | C250T |
| 14 | Liver | 22 | TMB-low | MSS | C228T | 38.1 | 578 | 60 | C228T |
| 15 | Liver | 47 | TMB-low | MSS | C250T | 12.0 | 875 | 60 | C250T |
| 16 | Liver | 35 | TMB-low | MSS | C228T | 56.2 | 441 | 80 | C228T |
| 17 | Liver | 153 | TMB-low | MSS | C228T | 28.6 | 398 | 70 | C228T |
| 18 | Liver | 83 | TMB-low | MSS | C228T | 24.4 | 586 | 80 | C228T |
| 19 | Liver | 135 | TMB-high | MSS | C250T | 38.2 | 728 | 80 | C250T |
| 20 | Liver | 121 | TMB-low | MSS | C228T | 34.7 | 254 | 60 | C228T |
| 21 | Liver | 140 | TMB-low | MSS | C228T | 20.9 | 134 | 70 | C228T |
| 22 | Liver | 139 | TMB-low | MSS | C228T | 49.4 | 237 | 60 | C228T |
| 23 | Melanoma | 50 | TMB-high | MSS | C250T | 11.4 | 500 | 60 | C250T |
| 24 | Melanoma | 98 | TMB-low | MSS | C250T | 28.8 | 351 | 80 | C250T |
| 25 | Melanoma | 52 | TMB-high | MSS | C228T | 23.8 | 395 | 40 | C228T |
| 26 | Melanoma | 76 | TMB-low | MSS | C228T | 54.6 | 227 | 70 | C228T |
| 27 | Melanoma | 76 | TMB-low | MSS | C228T | 26.9 | 93 | 70 | C228T |
| 28 | Melanoma | 190 | TMB-low | MSS | C250T | 41.5 | 585 | 40 | C250T |
| 29 | Melanoma | 138 | TMB-high | MSS | C250T | 57.5 | 315 | 30 | C250T |
| 30 | Melanoma | 49 | TMB-low | MSS | C228T | 53.4 | 251 | 80 | C228T |
| 31 | Melanoma | 195 | TMB-high | MSS | C250T | 21.7 | 359 | 80 | C250T |
| 32 | Melanoma | 137 | TMB-low | MSS | C228T | 20.8 | 226 | 50 | C228T |
| 33 | Melanoma | 138 | TMB-low | MSS | C250T | 29.3 | 399 | 40 | C250T |
| 34 | Melanoma | 136 | TMB-low | MSS | C250T | 47.5 | 385 | 40 | C250T |
| 35 | Pancreatobiliary | 188 | TMB-low | MSS | C228T | 34.6 | 81 | 60 | C228T |
| 36 | Pancreatobiliary | 456 | TMB-high | MSS | C228T | 20.5 | 234 | 50 | C228T |
| 37 | Pancreatobiliary | 50 | TMB-low | MSS | C228T | 12.1 | 239 | 50 | C228T |
| 38 | Pancreatobiliary | 61 | TMB-low | MSS | C228T | 10.8 | 249 | 25 | C228T |
| 39 | Pancreatobiliary | 159 | TMB-low | MSS | C228T | 11.7 | 137 | 10 | C228T |
| 40 | Pancreatobiliary | 15 | TMB-low | MSS | C228T | 33.1 | 130 | 90 | C228T |
| 41 | Pancreatobiliary | 17 | TMB-high | MSS | C228T | 31.7 | 145 | 70 | C228T |
| 42 | Pancreatobiliary | 19 | TMB-low | MSS | C250T | 16.9 | 705 | 40 | C250T |
| 43 | Pancreatobiliary | 57 | TMB-low | MSS | C228T | 36.6 | 544 | 50 | C228T |
| 44 | Pancreatobiliary | 11 | TMB-low | MSS | C228T | 45.9 | 270 | 60 | C228T |
| 45 | Pancreatobiliary | 22 | TMB-high | MSS | C250T | 18.0 | 666 | 60 | C250T |
| 46 | Pancreatobiliary | 45 | TMB-low | MSS | C228T | 21.7 | 337 | 30 | C228T |
| 47 | Pancreatobiliary | 41 | TMB-low | MSS | C228T | 29.3 | 246 | 30 | C228T |
| 48 | Pancreatobiliary | 18 | TMB-low | MSS | C250T | 12.8 | 639 | 60 | C250T |
| 49 | Pancreatobiliary | 23 | TMB-high | MSS | C228T | 25.1 | 470 | 40 | C228T |
| 50 | Pancreatobiliary | 36 | TMB-high | MSS | C228T | 24.9 | 503 | 70 | C228T |
| 51 | Pancreatobiliary | 36 | TMB-low | MSS | C228T | 19.1 | 236 | 40 | C228T |
| 52 | Pancreatobiliary | 11 | TMB-low | MSS | C228T | 33.1 | 366 | 30 | C228T |
| 53 | Pancreatobiliary | 47 | TMB-high | MSS | C228T | 39.2 | 199 | 80 | C228T |
| 54 | Urinary | 20 | TMB-low | MSS | C228T | 32.6 | 331 | 70 | C228T |
| 55 | Urinary | 81 | TMB-low | MSS | C228T | 22.7 | 238 | 70 | C228T |
| 56 | Urinary | 74 | TMB-low | MSS | C250T | 16.5 | 388 | 60 | C250T |
| 57 | Urinary | 172 | TMB-high | MSS | C250T | 19.7 | 117 | 70 | C250T |
| 58 | Urinary | 28 | TMB-high | MSS | C228T | 32.8 | 125 | 80 | C228T |
| 59 | Urinary | 30 | TMB-high | MSS | C228T | 19.5 | 41 | 30 | C228T |
| 60 | Urinary | 32 | TMB-high | MSS | C228T | 30.9 | 628 | 80 | C228T |
| 61 | Urinary | 70 | TMB-low | MSS | C228T | 21.7 | 60 | 60 | C228T |
| 62 | Urinary | 24 | TMB-high | MSS | C250T | 8.7 | 69 | 10 | C250T |
| 63 | Urinary | 35 | TMB-low | MSS | C250T | 22.3 | 197 | 70 | C250T |
| 64 | Urinary | 58 | TMB-high | MSS | C228T | 37.9 | 103 | 60 | C228T |
| 65 | Urinary | 54 | TMB-low | MSS | C228T | 14.2 | 106 | 60 | C228T |
| 66 | Urinary | 31 | TMB-high | MSS | C250T | 24.7 | 515 | 70 | C250T |
| 67 | Urinary | 47 | TMB-high | MSS | C228T | 28.1 | 740 | 70 | C228T |
| 68 | Urinary | 38 | TMB-high | MSS | C228T | 46.9 | 260 | 40 | C228T |
| 69 | Urinary | 43 | TMB-low | MSS | C250T | 49.2 | 1140 | 90 | C250T |
| 70 | Urinary | 25 | TMB-low | MSS | C250T | 47.6 | 993 | 70 | C250T |
| 71 | Urinary | 49 | TMB-low | MSS | C228T | 34.3 | 134 | 40 | C228T |
| 72 | Urinary | 22.4 | TMB-low | MSS | C228T | 53.8 | 409 | 35 | C228T |
| 73 | Urinary | 32 | TMB-low | MSS | C228T | 10.8 | 510 | 50 | C228T |
| 74 | Urinary | 27 | TMB-low | MSS | C228T | 25.3 | 301 | 20 | C228T |
| 75 | Urinary | 63 | TMB-low | MSS | C228T | 18.4 | 87 | 90 | C228T |
| 76 | Urinary | 42 | TMB-low | MSS | C228T | 47.6 | 410 | 70 | C228T |
| 77 | Urinary | 38 | TMB-high | MSS | C228T | 24.6 | 272 | 20 | C228T |
| 78 | Urinary | 63 | TMB-low | MSS | C228T | 21.1 | 95 | 70 | C228T |
| 79 | Urinary | 51 | TMB-high | MSS | C228T | 37.1 | 329 | 40 | C228T |
| 80 | Urinary | 169 | TMB-low | MSS | C228T | 30.1 | 332 | 80 | C228T |
| 81 | Urinary | 30 | TMB-low | MSS | C228T | 27.9 | 219 | 70 | C228T |
| 82 | Urinary | 15 | TMB-low | MSS | C228T | 18.1 | 205 | 40 | C228T |
| 83 | Urinary | 27 | TMB-low | MSS | C228T | 32.1 | 545 | 40 | C228T |
| 84 | Urinary | 39 | TMB-low | MSS | C228T | 18.1 | 276 | 70 | C228T |
| 85 | Brain | 51 | TMB-low | MSS | C228T | 51.6 | 31 | 70 | C228T |
| 86 | Colon | 64 | TMB-high | MSS | C228T | 28.3 | 152 | 40 | C228T |
| 87 | Colon | 76 | TMB-low | MSS | C228T | 29.4 | 286 | 50 | C228T |
| 88 | Colon | 87 | TMB-low | MSS | C228T | 31.8 | 63 | 90 | C228T |
| 89 | Colon | 61 | TMB-high | MSS | C228T | 34.3 | 429 | 60 | C228T |
| 90 | Colon | 18 | TMB-low | MSS | C228A | 38.8 | 268 | 40 | C228A |
| 91 | Colon | 30 | TMB-low | MSS | C228T | 38.9 | 779 | 30 | C228T |
| 92 | Colon | 42 | TMB-low | MSS | C228A | 53.2 | 139 | 60 | C228A |
| 93 | Head and neck | 54 | TMB-low | MSS | C228T | 25.3 | 95 | 20 | C228T |
| 94 | Head and neck | 66 | TMB-low | MSS | C228T | 31.6 | 493 | 70 | C228T |
| 95 | Head and neck | 77 | TMB-low | MSS | C250T | 85.1 | 215 | 50 | C250T |
| 96 | Lung | 90 | TMB-low | MSS | C228T | 20.2 | 134 | 60 | C228T |
| 97 | Lung | 37 | TMB-high | MSS | C228T | 26.2 | 84 | 40 | C228T |
| 98 | Sarcoma | 26 | TMB-low | MSS | C228T | 50.0 | 118 | 90 | C228T |
| 99 | Sarcoma | 14 | TMB-low | MSS | C228T | 56.6 | 53 | 80 | C228T |
| 100 | Sarcoma | 37 | TMB-low | MSS | C250T | 67.5 | 437 | 50 | C250T |
| 101 | Sarcoma | 45 | TMB-low | MSS | C228T | 70.9 | 117 | 25 | C228T |
| 102 | Skin | 136 | TMB-low | MSS | C228T | 37.8 | 394 | 20 | C228T |
| 103 | Thyroid | 163 | TMB-low | MSS | C228T | 45.6 | 204 | 80 | C228T |
NGS next-generation sequencing, TERT telomerase reverse transcriptase, VAF variant allele frequency, TD total read depth, TV tumor volume