Table 3.

Comparison of iTERT PCR with NGS

	NGS	iTERT PCR
Quality of DNA	Limited by damaged DNA in certain cases Needed high-quality DNA	Rarely limited by damaged DNA
Quantity of DNA	Needed the amount of DNA required for downstream NGS preparation steps (50 ~ 120 ng)	Relatively 'small' amount of DNA is required (< 50 ng)
Test time	Requires more time for the preparation of library preparation (2 days)	Time-saving and easy PCR preparation (< 3 h)
Costs (per case)	£570	£30
Interpretation	Very complex, and its interpretation requires expert bioinformatics assistance	Easy to analyze PCR-Sanger sequencing results