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. 2022 Jan 28;16(1):e0010180. doi: 10.1371/journal.pntd.0010180

Fig 1. Workflow of ETEC and Shigella RLDT using RLDT kit.

Fig 1

Step 1, using the fixed volume pipette provided in the kit, add a stool sample to the Sample Processing Tube (SPT) with lysis buffer. Step 2, Cap the SPT tube and mix by up and down. Squeeze the SPT tube to add the lysis buffer + stool sample to the lysis tube (1.5ml microfuge tube). Step 3, lysis tube is heated at 90°C for 5 minutes in a heat block or water bath. Step 4, add 25 μL of lysate to each tube of the lyophilized reaction tubes (LRT) strip using disposable fixed volume pipette. Step 5, place the LRTs in the reader. Scan the barcode for ETEC and Shigella RLDT program. Step 6, read results displayed on the screen after 40 minutes. All the reagents and sample processing accessories required for RLDT are included in the kit. Illustration was created with BioRender.com.