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. 2022 Feb 9;16(2):e0010170. doi: 10.1371/journal.pntd.0010170

Fig 6. Characterization of the inheritance of extra chromosome sets in the interspecies hybrid clones.

Fig 6

(A) Agarose gels of the SNP-CAPs analysis showing the DNA products after PCR amplification and enzymatic digestion of three loci present in chromosomes 2, 22 and 35. The top panel shows the electrophoretic profile of L. donovani mCh HYG (Ld), L. major CTN PAC (Lm) and three hybrids (Ld x Lm), and the ratios between the intensity of the upper band from the parental L. donovani and the uncut upper band from the other parental L. major in the corresponding hybrid clones. Simulated 3n were created by mixing parental DNA in 1:1, 1:2 and 2:1 ratio (L. donovani:L. major). The bottom panel shows the electrophoretic profile of L. donovani mCh HYG (Ld), L. tropica iRFP PAC (Lt) and two hybrids (Ld x Lt), and the ratios between the intensity of the upper band from the parental L. donovani and the uncut upper band from the other parental L. tropica in the corresponding hybrid clones. Simulated 4n were created by mixing parental DNA in 1:1, 1:3 and 3:1 ratio (L. donovani:L. tropica). The arrowhead indicates 1000 bp in the DNA molecular weight marker (M). (B, C) Statistical representation by k-means analysis in SPSS of the extrachromosome inheritance of L. donovani mCh HYG x L. major CTN PAC (Ld mCh HYG x Lm CTN PAC) hybrids, which are clustered together with ratio 2:1 (LdxLm) (B), and L. donovani mCh HYG x L. tropica iRFP PAC (Ld mCh HYG x Lt iRFP PAC) hybrids, the latter being clustered together with ratio 1:3 (LdxLt) (C).