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. 2022 Feb 9;16(2):e0010170. doi: 10.1371/journal.pntd.0010170

Fig 7. Characterization of the inheritance of kDNA in the interspecies hybrid clones.

Fig 7

(A, B, top panels) Agarose gels of the SNP-CAPs analysis showing the DNA products after PCR amplification and enzymatic digestion of Cyb and ND5 genes. The electrophoretic profile of the parental lines [P (Ld, Lm)] and three hybrids [Hybrids (1, 2, 3)] resulting from the outcrossing of L. donovani mCh HYG (Ld) x L. major CTN PAC (Lm) (A), or the parental lines [P (Ld, Lt)] and two hybrids [Hybrids (1, 2)] resulting from the outcrossing L. donovani mCh HYG (Ld) x L. tropica iRFP PAC (Lt) (B), are shown. (A, B bottom panels) Fluorograms showing the sequence of part of the ND7 and 12 sRNA genes with some SNPs, which have been indicated as Ld (L. donovani) and Lm (L. major) in the parental strains L. donovani mCh HYG (Ld), L. major CTN PAC (Lm) and in their clonal hybrids (LdxLm) 1, 2 and 3 (A), or as Ld (L. donovani) and Lt (L. tropica) in the parental strains L. donovani mCh HYG (Ld), L. tropica iRFP PAC (Lt) and in their clonal hybrids (LdxLt) 1 and 2 (B). (C) Agarose gels showing the band profile after amplification of a conserved region (MIN1) or a variable region (MIN2) of minicircles in the parental lines [P (Ld, Lm)] and three hybrids [Hybrids (1, 2, 3)] resulting from the outcrossing of L. donovani mCh HYG (Ld) x L. major CTN PAC (Lm) (left panel), or in the parental lines [P (Ld, Lt)] and two hybrids [Hybrids (1, 2)] resulting from the outcrossing L. donovani mCh HYG (Ld) x L. tropica iRFP PAC (Lt) (right panel). The arrowhead indicates 1000 bp in the DNA molecular weight marker (M).