Table 1. Primers used in this work.
| Product name | Primers | Product size (bp) | Use |
|---|---|---|---|
| Citrine [25] | RBF921, FW: ccgCTCGAGgaAGATCTCCACCATGGTGAGCAAGGGCGAGG RBF614, RV: ataagaatGCGGCCGCTTACTTGTACAGCTCGTCCATG |
720 | ORF cloning |
| Hygromycine [33] | RBF646, FW: ATGAAAAAGCCTGAACTCACC RBF647, RV: CTATTCCTTTGCCCTCGGAC |
1025 | Characterization of transformants |
| Puromycin [32] | RBF774, FW: ATGACCGAGTACAAGCCCACG RBF775, RV: TCAGGCACCGGGCTTGCGG |
720 | Characterization of transformants |
| mCherry | TOX43, FW: TGGCCATCATCAAGGAGTTCA TOX44, RV: CCCTCGGCGCGTTCGT |
711 | Characterization of transformants |
| Citrine | TOX41, FW: CGAGGAGCTGTACACCGGG TOX42, RV: ACGAACTCCAGCAGGACCAT |
717 | Characterization of transformants |
| iRFP [32] | RBF843, FW: ATGGCGGAAGGATCCGTCGC RBF844, RV: TCACTCTTCCATCACGCCGATC |
950 | Characterization of transformants |
| Calmodulin-binding | TOX59, FW: CTGAACCGGATGCAGATTCGTGAG TOX60, RV: CTTGTTGCGTGTCGTCTCGATCTG |
786 | Genomic SNP-CAPs |
| ABC transporter | TOX61, FW: ATGGACTTGGTGCAGCTGCAGCGA TOX62, RV: GATGTCATCGCACAGGGACGTAATG |
2091 | Genomic SNP-CAPs |
| Sec20 | TOX63, FW: GGACCAGGCGCTACACGAGCTGAT TOX64, RV: GACACGCGCAGCAGCAGATCATCG |
618 | Genomic SNP-CAPs |
| Rad9 | TOX65, FW: GGCTGAGCGACGGCATCGAGATCAG TOX66, RV: CCTCGTTGGCGGAGGTGTAGGTGC |
1956–1959 | Genomic SNP-CAPs |
| Asparaginase | TOX69, FW: CGTCGCCGGATACCTGACGGAGC TOX70, RV: GGTGATTTCGCCTCGCAGATTCTG |
1015 | Genomic SNP-CAPs |
| Paraflagellar rod protein | TOX67, FW: ATGAGCATCGCTGCGGACATGGCGT TOX68, RV: CTACTCGGTGATCTGTCGCACCGTC |
1800 | Genomic SNP-CAPs |
| Ribosomal protein L22p/L17e | TOX55, FW: ATGCCGAAGCCAGCCCCTCGGTAC TOX56, RV: CAGCAGTGATGGCGGGCACGTCCA |
829 | Genomic SNP-CAPs |
| Fumarate hydratase (Fumerase) | TOX45, FW: ATGTCTCTGTGCGACCA TOX46, RV: TCACGCAAGCGTCTTCG |
1707 | Genomic SNP-CAPs |
| Aquaglyceroporin 1 | TOX51, FW: GGCTACGCGAGTATGTTGCC TOX52, RV: AAGACATACAAGAACATGCCGA |
688–736 | Genomic SNP-CAPs |
| 2-oxoisovalerate dehydrogenase beta subunit | TOX49, FW: AGCGTTCAACCGAGTGAGTT TOX50, RV: AAGTGCACCACGGACTTGAT |
1025 | Genomic SNP-CAPs |
| A2 [36] | L2: TTGGCAATGCGAGCGTCACAGTC R3: CAACGCGTACGATAATGCCACA |
230/480 | Genomic amplification |
| ACTIN | TOX83, FW: AATGGCTGACAACGAGCAGA TOX84, RV: CACTTGTTGTGCACGATGCT |
1125 | Genomic sequencing |
| ITS [37] | (LITSR); TOX75, FW: CTGGATCATTTTCCGATG (L5.8S); TOX76, RV: TGATACCACTTATCGCACTT |
420 | Strain confirmation |
| ND7 [38] | TOX97, FW: GTGCATTTATGCGTTTATTAATGTG TOX98, RV: ACAACATCAACATTACCAATAACTGC |
800 | Maxicircle SNP-CAPs |
| CYTB [26] | TOX99, FW: AGCGGAGAGRARAGAAAAGG TOX100, RV:: GYTCRCAATAAAATGCAAATC |
618 | Maxicircle SNP-CAPs |
| ND5 [26] | TOX103, FW: GAYGCDATGGAAGGACCDAT TOX104, RV: CCACAYAAAAAYCAYAANGAACA |
456 | Maxicircle SNP-CAPs |
| 12sRNA [26] | TOX101, FW: AACTARTGAWGGCACAGTTGTTCT TOX102, RV: ACCCAACTAACGAATTGCWTTT |
818 | Maxicircle SNP-CAPs |
| MINICIRCLES 1 (MIN 1) [39] | TOX73, FW: CCAGTTTCCCGCCCCG (KDNA-F) TOX74, RV: GGGGTTGGTGGTGTAAAATAG (KDNA-R) |
780 | Minicircle amplification |
| MINICIRCLES 2 (MIN 2) [40] | TOX75, FW: TAATATAGTGGGCCGCGCAC TOX76, RV: CCGACATGCCTCTGGGTAGG |
<250–1000 | Minicircle amplification |