Table 1. Cryo-EM data collection, refinement, and validation statistics.
RMS, root mean square; EMDB, Electron Microscopy Data Bank.
|
Data collection
and processing |
Primmed vault |
Committed
vault |
Half vault |
| Magnification | ×81,000 | ×130,000 | ×81,000 |
| Voltage (kV) | 300 | 300 | 300 |
| Electron exposure (e−/Å2) |
30 | 35 | 30 |
| Defocus range (μm) |
−1.5/−3.5 | −1.5/−3.5 | −1.5/−3.5 |
| Pixel size (Å) | 1.78 | 1.07 | 1.78 |
| Symmetry imposed |
D39 | C1 | C1 |
| Initial particle images (n) |
34,822 | 207,815 | 34,822 |
| Final particle images (n) |
9793 | 11,666 | 7539 |
| Map resolution (Å) |
3.7 | 7.9 | 9.3 |
| FSC threshold | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) |
3–8 | 7–13 | 8–14 |
| Refinement | |||
| Initial model used (PDB code) |
4HL8 | – | – |
| B factors (Å2) | −156.45 | – | – |
| RMS deviations | |||
| Bond lengths (Å) |
0.07 | – | – |
| Bond angles (°) | 0.81 | – | – |
| Validation | |||
| MolProbity score |
20.27 | – | – |
| Ramachandran plot |
|||
| Favored (%) | 88.24 | – | – |
| Allowed (%) | 11.25 | – | – |
| Outliers (%) | 0.52 | – | – |
| Databases | |||
| EMDB | EMD-13478 | EMD-13483 | EMD-13482 |
| PDB | 7PKR | – | – |