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(a) Gel permeation chromatography of the 30 to 70% ammonium sulfate fraction of A. lumbricoides SAg on a Sephacryl S-300 column. (b) Separation of Als III on a Resource-Q anion exchanger by FPLC. (c) Purification of Als IIIb by HPGPLC on a protein PAC 300 SW column. Abs., absorbance.
