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. 2022 Feb 9;11:e72182. doi: 10.7554/eLife.72182

Figure 1. Celastrol ameliorates adipose hypertrophy and lipid metabolism.

Figure 1.

(A, B) Hematoxylin and eosin (H&E) staining of mouse hepatic tissues and epididymal fat pads. After 21-day treatment with vehicle or celastrol, the livers and epididymal fat pads were recovered from four groups of C57/BL6 mice (i.e., Control, HFD, HFD + C5, HFD + C7.5) and stained with H&E stain. Representative images were shown. Scale bar represented 34 μm in length. (C, D) 3D gas chromatography–mass spectrometry (GC–MS) chromatograms of free fatty acids from murine livers and epididymal fat pads. After 21-day treatment with vehicle or celastrol, livers and epididymal fat pads were recovered from four groups of C57/BL6 mice (i.e., Control, HFD, HFD + C5, HFD + C7.5) for fatty acids extraction and GC–MS profiling. (E, F) GC–MS profiling of fatty acids from murine livers and epididymal fat pads. Free and conjugated fatty acids were profiled by GC–MS. Total amounts of individual fatty acids were quantified. The results were presented as mean ± standard deviation (SD) of three independent experiments. a, p < 0.05; b, p < 0.01; c, p < 0.001.

Figure 1—source data 1. Data for Figure 1A–D.
Figure 1—source data 2. Data for Figure 1E, F.