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. 2022 Feb 9;11:e72182. doi: 10.7554/eLife.72182

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© 2022, Luo et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (A) One-week effects of celastrol on the biomarkers of lipid metabolism in liver tissues. After 7-day treatment, total RNAs were extracted from livers and analyzed by quantitative real-time PCR (qRT-PCR) technique using Qiagen QuantiTect SYBR Green PCR Kit and specific DNA primers. N = 3. (B) Three-week effects of celastrol on the biomarkers in lipid metabolism in liver tissues. After 21-day treatment, total RNAs were extracted from livers and analyzed by qRT-PCR technique using Qiagen QuantiTect SYBR Green PCR Kit and specific DNA primers. N = 3. (C) Three-week effects of celastrol the biomarkers in lipid metabolism in epididymal adipose tissues. After 21-day treatment, total RNAs were extracted from epididymal adipose tissues and analyzed by qRT-PCR technique using Qiagen QuantiTect SYBR Green PCR Kit and specific DNA primers. N = 3. HFD, HFD only; C5.0, celastrol (5 mg kg⁻¹ d⁻¹); C7.5, celastrol (7.5 mg kg⁻¹ d⁻¹); a, p < 0.05; b, p < 0.01; c, p < 0.001.

Figure 2—source data 1. Data for Figure 2A–C.

elife-72182-fig2-data1.xlsx^{(13.5KB, xlsx)}