| Strain, strain background (Mus musculus, male/female) |
C57BL/6 |
Shanghai SLAC Laboratory Animal C.,Ltd |
|
|
| Strain, strain background (Mus musculus, male/female) |
kif2cflox/flox mouse |
Model Animal Research Center of Nanjing University |
|
|
| Strain, strain background (Mus musculus, male/female) |
NestinCre mouse |
Zhejiang University |
Wang et al., 2019
|
|
| Cell line (Homo-sapiens) |
HEK 293T |
ATCC |
CRL-3216 |
|
| Genetic reagent (virus) |
pAKD-CMV-bGlobin-mcherry-H1-shRNA |
ObioTechnology |
|
|
| Genetic reagent (virus) |
pAAV-CMV-KIF2C-3FLAG-P2A-mNeonGreen-CW3SL |
ObioTechnology |
|
|
| Genetic reagent (virus) |
pAAV-CMV-KIF2C(G491A)–3FLAG-P2A-mNeonGreen-CW3SL |
ObioTechnology |
|
|
| Genetic reagent (virus) |
rAAV-TRE3G- KIF2C-3*Flag –2A-mCherry-WPRE-pA |
BrainVTA |
|
|
| Antibody |
Anti-KIF2C, (rabbit polyclonal) |
Proteintech |
Cat# 12139–1-AP, RRID:AB_2877829
|
IF (1:500), WB (1:500) |
| Antibody |
Anti-PSD-95, (mouse monoclonal) |
Abcam |
Cat# ab2723, RRID:AB_303248
|
IF (1:1000) WB (1:1000) |
| Antibody |
Anti-MAP2, (chicken polyclonal) |
Abcam |
Cat# ab5392, RRID:AB_2138153
|
IF (1:2000) |
| Antibody |
anti-GFP (rabbit polyclonal) |
Abcam |
Cat# ab6556, RRID:AB_305564
|
IF (1:2000) |
| Antibody |
Anti-α-tubulin, (rabbit monoclonal) |
Abcam |
Cat# ab52866, RRID:AB_869989
|
IF (1:1000) |
| Antibody |
Anti-mCherry, (rabbit polyclonal) |
Abcam |
Cat# ab167453, RRID:AB_2571870
|
WB (1:2000) |
| Antibody |
Anti-EB3, (rabbit monoclonal) |
Abcam |
Cat# ab157217, RRID:AB_2890656
|
WB (1:10,000) |
| Antibody |
Anti-α-tubulin, tyrosinated, (rat monoclonal) |
Millipore |
Cat# MAB1864-I, RRID:AB_2890657
|
IF (1:1000) |
| Antibody |
Anti-synaptophysin, (mouse monoclonal) |
Millipore |
Cat# MAB368, RRID:AB_94947
|
IF (1:1000) WB (1:2000) |
| Antibody |
Anti-GAPDH, (mouse monoclonal) |
Millipore |
Cat# MAB374, RRID:AB_2107445
|
WB (1:10,000) |
| Antibody |
Anti-GluA1, (rabbit monoclonal) |
Millipore |
Cat# 04–855, RRID:AB_1977216
|
WB (1:1000) |
| Antibody |
Anti-GluA2, (mouse monoclonal) |
Millipore |
Cat# MAB397, RRID:AB_2113875
|
WB (1:2000) |
| Antibody |
Anti-GluN2A, (rabbit polyclonal) |
Cell Signaling Technology |
Cat# 4205, RRID:AB_2112295
|
WB (1:2000) |
| Antibody |
Anti- GluN2B, (rabbit polyclonal) |
Cell Signaling Technology |
Cat# 4207, RRID:AB_1264223
|
WB (1:2000) |
| Antibody |
Anti-GluN1, (rabbit polyclonal) |
Cell Signaling Technology |
Cat# 5704, RRID:AB_1904067
|
WB (1:2000) |
| Antibody |
Anti-flotillin, (mouse monoclonal) |
BD Bioscience |
Cat# 610820, RRID:AB_398139
|
WB (1:1000) |
| Antibody |
Anti-β-tubulin, a (mouse monoclonal) |
Santa Cruz |
Cat# sc-5274, RRID:AB_2288090
|
WB (1:2000) |
| Antibody |
Anti- bassoon, (mouse monoclonal) |
Enzo Life Sciences |
Cat# ADI-VAM-PS003-F, RRID:AB_11181058
|
IF (1:1000) |
| Antibody |
Anti- N-cadherin, (rabbit polyclonal) |
Bioworld Technology |
Cat# BS2224, RRID:AB_1664028
|
WB (1:1000) |
| Chemical compound, drug |
CNQX |
Sigma-Aldrich |
Cat# C239; CAS: 115066-14-3 |
20 μM |
| Chemical compound, drug |
AP-V |
Sigma-Aldrich |
Cat# A8054; CAS: 79055-68-8 |
50 μM |
| Chemical compound, drug |
Doxycycline Hyclate |
Selleck |
Cat# S4163; CAS:24390-14-5 |
40 μg/ml |
| Chemical compound, drug |
Glycine |
Sigma-Aldrich |
Cat# G8898; CAS:56-40-6 |
200 μM |
| Chemical compound, drug |
bicuculline |
Abcam |
Cat# ab120108; CAS: 40709-69-1 |
20 μM |
| Chemical compound, drug |
Picrotoxin |
Sigma-Aldrich |
Cat# P1675; CAS: 124-87-8 |
100 μM |
| Commercial assay or kit |
SYBR Green qPCR Master Mix |
QIAGEN |
Cat# 204,057 |
|
| Commercial assay or kit |
Nissl staining Kit |
Beyotime |
Cat# C0117 |
|
| Commercial assay or kit |
FD Rapid GolgiStain Kit |
FD NeuroTechnologies |
Cat# PK401 |
|
| Commercial assay or kit |
BCA Protein Assay Kit |
Thermo Fisher |
Cat# 23,227 |
|
| Software, algorithm |
GraphPad Prism (8.0) |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/
|
RRID: SCR_002798
|
| Software, algorithm |
ImageJ |
National Institutes of Health |
https://imagej.nih.gov/ij/
|
RRID:SCR_003070
|
| Software, algorithm |
ANY-maze tracking software |
Stoelting Co |
https://www.stoeltingco.com/anymaze.html
|
RRID:SCR_014289
|
| Software, algorithm |
PlusTipTracker |
UTSouthwestern Medical Center Danuser Lab |
https://www.utsouthwestern.edu/labs/danuser/software/
|
|
| Software, algorithm |
Sigmaplot |
systatsoftware |
http://www.systatsoftware.cn/
|
RRID:SCR_003210
|
| Software, algorithm |
MATLAB R2017b |
MathWorks |
https://se.mathworks.com/products/matlab.html
|
RRID: SCR_001622
|
| Sequence-based reagent |
KIF2C floxP _F |
This paper |
PCR primers |
GGT CCA GCT CTT TAC TGA TGT GTT C |
| Sequence-based reagent |
KIF2C floxP _R |
This paper |
PCR primers |
ACA AAG CAA GTC CAG GTC CAA G |
| Sequence-based reagent |
Nestin-Cre_F |
This paper |
PCR primers |
TGC AAC GAG TGA TGA GGT TC |
| Sequence-based reagent |
Nestin-Cre_R |
This paper |
PCR primers |
GCT TGC ATG ATC TCC GGT AT |
| Sequence-based reagent |
kif2c_F |
This paper |
PCR primers |
TGC CGT TGT TGA TGG TCA GTG |
| Sequence-based reagent |
kif2c_R |
This paper |
PCR primers |
GGA GAC ACT TGC TGG GAA CAG |
| Sequence-based reagent |
shkif2c_F |
This paper |
PCR primers |
TGG ATC GAA GGA GGT ACC AC |
| Sequence-based reagent |
shkif2c_R |
This paper |
PCR primers |
CAC TGA CCA TCA ACA ACG GCA |
| Sequence-based reagent |
gapdh_F |
This paper |
PCR primers |
AGG TCG GTG TGA ACG GAT TTG |
| Sequence-based reagent |
gapdh_R |
This paper |
PCR primers |
TGT AGA CCA TGT AGT TGA GGT CA |
| Peptide, recombinant protein |
BDNF |
Sigma-Aldrich |
Cat# GF301 |
50 ng/mL |
