TABLE 1.
Digestion time, incubation temperature, and discriminatory ability of 15 restriction enzymes used for characterization of 16 L. monocytogenes isolates
| Enzyme | Incubation temp (°C) | Digestion time (min) | No. of ribotypes among 16 isolates | SID |
|---|---|---|---|---|
| PvuII | 37 | 20 | 15 | 0.992 |
| EcoRI | 37 | 20 | 12 | 0.950 |
| BstEII | 60 | 120 | 10 | 0.925 |
| BanI | 37 | 120 | 10 | 0.917 |
| XhoI | 37 | 120 | 10 | 0.900 |
| BglII | 37 | 120 | 8 | 0.883 |
| EagI | 37 | 120 | 9 | 0.883 |
| AseI | 37 | 120 | 7 | 0.817 |
| HincII | 37 | 120 | 5 | 0.792 |
| PstI | 37 | 120 | 6 | 0.617 |
| NcoI | 37 | 120 | 4 | 0.617 |
| XbaI | 37 | 120 | 1 | 0 |
| BsoBI | 60 | 120 | NDa | ND |
| ClaI | 37 | 120 | ND | ND |
| StyI | 37 | 120 | ND | ND |
a
ND, not determined. The enzymes BsoBI, ClaI, and StyI showed incomplete digests with spurious and variable high-molecular-weight bands, which did not allow assignment of distinct ribotypes.