FIGURE 1.

Evaluation of feeder cell residues in hPSC mRNA‐Seq data sets. (A) Strategy for identification of species‐specific reads in mRNA‐Seq. (B) Ratio of mouse‐derived reads in mRNA‐Seq data sets of hPSCs maintained in different cultured systems. (C) Expression pattern of mESC, hepatocyte and MEF marker genes in mouse‐specific read sets separated from hPSC mRNA‐Seq data sets. (D) Correlation analysis for the whole genome‐wide gene expression pattern of mouse‐specific read sets separated from hPSC mRNA‐Seq of the ESC, hepatocyte and MEF data sets. (E) Human genes whose expression levels might be influenced by reads derived from residual mouse feeder cells