FIGURE 3.

Assessment of feeder cell residues in hPSC miRNA‐Seq data sets. (A) Specifically expressed miRNAs or miRNA clusters in feeder cells compared to low and high passaged hECSs. The miRNA expression level (RPM) was normalized by log2. (B) Heatmap showing the expression levels of marker miRNAs in published hESCs cultured in a feeder system. The marker types are listed on the top‐right and the miRNAs are listed in Table S2. (C) Specific miRNA residual levels of feeder cells in H9 hESCs isolated by different methods. (D) Conserved miRNAs between human and mouse whose expression levels might be influenced by reads derived from residual mouse feeder cells