Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 25;602(7896):300–306. doi: 10.1038/s41586-021-04266-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — The GFP-expressing D614G and D614G/P681R mutant viruses were generated by reverse genetics. These viruses (100 TCID₅₀ for Vero and VeroE6/TMPRSS2 cells, 100 or 1,000 TCID₅₀ for Calu-3 cells) were inoculated into cells. The viral RNA copy number of in the culture supernatant (a) and the level of GFP-positive cells (the percentage of GFP-positive cells for Vero and VeroE6/TMPRSS2 cells; the GFP intensity per well for Calu-3 cells) (b) are shown. Data are mean ± S.D. (c) Representative images of Calu-3 cells infected with GFP-expressing viruses (100 TCID₅₀). Areas enclosed with circles are enlarged in the right panels. Assays were performed in quadruplicate. Statistically significant differences (*P < 0.05) versus the D614G virus were determined by two-sided Student’s t test. NS, no statistical significance.