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. 2022 Jan 27;13:772446. doi: 10.3389/fimmu.2022.772446

Figure 3.

Figure 3

GLP-1R knockout affects the peritoneal exudate cells migration. PECs were collected from untreated WT and GLP-1R KO mice. 600 μl of DMEM with 10% FBS and MIP-2 (200ng/ml, PeproTech) was added to the lower chamber. After incubation for 4h in 37°C, migration was assessed by fixing the cells at the bottom of the transwell membrane with methanol followed by staining with crystal violet (0.1%, Sigma-Aldrich). (A) Representative pictures of the stained migrated cells at the bottom of the Transwell® membrane were taken using an inverted microscope (n=6). (B) Quantification of the number of PECs at the bottom of the transwell membrane(n=6). (C) Crystal violet was resolved with 33% acetic acid and the absorbance was measured by microplate reader at 570 nm (n=6). After subtracting the optical density (OD) value of blank well, the ODs of WT and KO wells are presented. Data is presented as mean ± SEM. The results shown are from one of three independent experiments. ***p < 0.001. ns, not significant.