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. 2022 Jan 20;17(2):259–275. doi: 10.1016/j.stemcr.2021.12.010

Figure 1.

Figure 1

Hippocampal neurogenesis and precursor number decline during aging

Also see Figure S1. Coronal DG sections from 6 week, 3 month, and 12 month old mice were analyzed by immunostaining.

(A and B) Sections were immunostained for DCX (A, green, arrows), and total DG DCX-positive cells were quantified (B).

(C) DG image from a BrdU-injected 6 week mouse analyzed 1 month later, immunostained for BrdU (green) and NeuN (red). White hatched box is shown at higher magnification at the bottom. Arrows denote double-positive cells.

(D) Quantification of sections as in (C) for total DG BrdU-positive, NeuN-positive neurons.

(E and F) Sections were immunostained for SOX2 (E, green, arrows), and total SGZ SOX2-positive cells were quantified (F).

(G and H) DG sections from 6 week (G) and older mice were immunostained for SOX2 (G, green) and KI67 (G, red; arrow denotes double-labeled cell), and the percentage of SOX2-positive SGZ cells that were also KI67 positive was quantified (H). Dashed line indicates the SGZ/hilus border.

(I and J) DG sections of 6 weeks (I) and older mice were immunostained for SOX2 (I, green) and ASCL1 (I, red; arrows denote double-positive cells), and the percentage of SOX2-positive SGZ cells that were also ASCL1 positive was quantified (J). Dashed line indicates the SGZ/hilus border.

(K and L) DG sections of 6 week (K) and older mice were immunostained for SOX2 (K, green) and GFAP (K, red; arrows denote double-positive cells), and total SGZ GFAP-positive, SOX2-positive cells were quantified (L).

(M and N) Six week (M) and 12-month DG sections were immunostained for GFAP (M, magenta), SOX2 (M, green), and KI67 (M, red; the arrow indicates a triple-positive cell, the arrowhead a SOX2-positive, GFAP-positive cell, and the double arrowhead a SOX2-positive, KI67-positive, GFAP-negative cell), and the percentage of GFAP-positive, SOX2-positive SGZ cells that were also KI67 positive was determined (N). In all cases, error bars indicate standard error of the mean (SEM), n = 3 mice/time point, and ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Sections in (A and E) were counterstained for Hoechst 33258 (blue). Scale bars: 50 μm (A and E), 30 μm (C), 20 μm (G and I), and 10 μm (K and M).