Figure 2.

H3K79me2 is enriched on numerous genes, yet few change transcriptionally in steady-state mRNA levels

(A) Genes upregulated (gold) or downregulated (blue) by DOT1Li treatment at any time point relative to the matched control (DOT1Li-DE list) plotted on expression calculated as log10 of ESC TPM (left) or MEF TPM (right) versus log2 FC in ESCs relative to MEFs.

(B) Genes with H3K79me2 called peaks in ESCs (red), in MEFs (green), shared in both cell types (orange), or with no peak (purple) were plotted onto the indicated expression calculated as log10 TPM versus log2 FC in ESCs relative to MEFs. H3K79me2 location data were analyzed from GEO: GSE90895 (Chronis et al., 2017).

(C) Bar graph of H3K79me2 called peaks at DOT1Li-DE genes. Significance calculated by Fisher's exact test is shown.

(D) Overlap of genes with an H3K79me2 peak on day 2 of reprogramming (Chronis et al., 2017) with DE genes at the indicated time points.

See also Figure S2.