Skip to main content
. 2022 Jan 6;17(2):369–383. doi: 10.1016/j.stemcr.2021.12.002

Figure 4.

Figure 4

Mice deficient for Rnf43 show no detectable changes in taste tissues and mild changes in the dorsal lingual epithelium

(A) Schematic illustration of the experimental design.

(B) Representative bright-field images of tongues collected from control and single Rnf43 KO mice.

(C) Immunofluorescence staining of anterior tongue sections of control and single Rnf43 KO mice for E-cadherin (ECAD, green) and KI-67 (red). Note that KI-67+ cells are fewer in Rnf43 KO mice than control mice. Cell nuclei were counterstained with DAPI (blue). Scale bars, 50 μm.

(D and E) Immunofluorescence staining for KRT8 (green) and P2X3 (red) of anterior tongue sections (D) and circumvallate papilla sections (E) from control and single Rnf43 KO mice. There are no apparent changes in the KRT8 and P2X3 staining patterns. Cell nuclei were counterstained with DAPI (blue). Scale bars, 50 μm.

(F) Number of KRT8+ taste buds in the circumvallate papilla from control and single Rnf43 KO mice. Data are presented as mean ± SEM. n.s., not significant. n = 4 for each group. Each point represents a single mouse. CvP, circumvallate papilla.