Figure 5.

CRISPRa + ME cells progress to the pluripotent state with improved fidelity

(A) Morphology of colonies during TG and CRISPRa + ME mid-reprogramming. Scale bar, 400 μm.

(B) Correlation coefficients of gene expression profile among cells in all mid-reprogramming conditions.

(C) Distribution of OCT4 expression level in cells from all mid-reprogramming conditions.

(D) Cell identity annotation in all mid-reprogramming conditions using SingleR. BM, bone marrow; CMP, common myeloid progenitors; GMP, granulocyte monocyte progenitors; MEP, megakaryocyte-erythroid progenitor cell.

(E) Flow cytometry analysis of hESC marker TRA-1-60 in TG and CRISPRa + ME mid-reprogramming cells. Red, negative control.

(F) Immunocytochemistry images showing TRA-1-60 expression in TG and CRISPRa + ME mid-reprogramming cells. Scale bar, 400 μm.