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. 2022 Jan 27;12:798243. doi: 10.3389/fpls.2021.798243

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Copyright © 2022 Ketumile, Yang, Sanchez, Kundariya, Rajewski, Dweikat and Mackenzie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sorghum dw3 reversion in Tx430. (A) Tx430 plants (dw3/dw3) in the field, with visible a DW3 revertant (red arrow). (B) Stalk internode compaction in the TX430-dwarfed dw3 phenotype (right) compared to a DW3 revertant (left). (C) DW3 revertants and Tx430 wild-type (dw3/dw3) genotypes assayed by PCR. Fragment size (bp) marker lane on left. Dw3 and dw3 alleles were discriminated by the presence of a tandem repeat of 882 bp in exon 5; the wild-type plants showed a major band (>1,500 bp). Polymerase chain reaction (PCR) conditions were optimized for amplification of revertant product. (D) MSH1-RNAi transgene-null (memory) plant containing the functional DW3 allele. Under the conditions of this study, DW3 was not detectable in the phenotype of MSH1-RNAi transgene-null (memory) plants.