Figure 3.

Siah2 regulates PD-L1 stability in CCA. (A, B), Depletion of Siah2 increases the protein level but not the mRNA level, of PD-L1 in CCA cells. (C), Depletion of Siah2 increases the protein stability of PD-L1 under CHX treatment. (D, E), Overexpression of Siah2 decreases the protein level, but not the mRNA level, of PD-L1 in CCA cells. (F), Overexpression of Siah2 decreases the protein stability of PD-L1 under CHX treatment. (G), The protein level of Siah2 was evaluated by Western blot in CCA cells with LV-NC or LV-METTL14 upon MG132 treatment. Mean ± SEM of three independent experiments. *P < 0.05; ^#P > 0.05.