Figure 6.

Siah2 enhanced in vivo antitumor T-cell activity. (A–C), CCA cells with LV-NC/LV-Siah2 were injected subcutaneously into the right flank of NCG mice to obtain tumor xenografts. Tumor volume (B) and tumor weight (C) were then measured. n = 5. ns, non-significant. Unpaired t test. (D), Western blot analysis detected PD-L1 level in the subcutaneous xenografts described in (A–C). (E–H), CCA cells with LV-NC/LV-Siah2 were injected subcutaneously into the right flank of NCG mice, which were adoptively transferred with activated HPBMC. (E–G), Images of subcutaneous xenografts (E), tumor volume (F), and tumor weight (G) are shown. N = 5. (H), FCM detected the percentage of human CD3⁺ T cells in HPBMC of NCG mice. n = 5. Mean ± SEM of three independent experiments. *P < 0.05; ^#P > 0.05.