Table 1.
Sensitivity of mammalian cells to WX8
| Cells (origin) | Cell line | WX8 IC50 (μM) |
||
|---|---|---|---|---|
| ATPa | Live Cellsb | DNAc | ||
| Mouse stem cells | ||||
| ESC (blastocyst) | Gmnn(fl/fl); ER-Cre/+ | 1.0 | Yes | |
| Human stem cells | ||||
| ESC (blastocyst) | WA01/H1 | 1.6 | 1.9 | 2 |
| ESC (blastocyst) | WA09/H9 | 0.26 | 2.5 | 5 |
| iPSC (H108 skin fibroblast) | SCUi24 | 0.4 | 2.5 | 1 |
| ECC (lung epithelial) | TERA-2 | 3 | 5 | 5 |
| ECC (testis epithelial) | NTERA-2 | 0.48 | 1.3/0.2 | 3.5/0.4 |
| ECC (testis epithelial) | GCT27D.C1 | 0.28 | 0.2 | |
| Differentiated cell lines | ||||
| Normal skin fibroblast | HFF-1 | 28 | 75/>10 | 50/>10 |
| Normal skin fibroblast | H108 | 15 | 50 | 140 |
| Normal lung epithelial | HBEC | 6 | 140 | 20 |
| Normal kidney epithelial | VeroE6 | 14 | 60 | 60 |
| Differentiated stem cells | ||||
| Differentiated ESCs | H1-diff | >10 | ||
| Differentiated iPSCs | SCUi24-diff | >10 | ||
| Teratocarcinoma xenografts | ||||
| ECC (testis epithelial) | NTERA-2 | Eliminated stem cells and inhibited tumor growth | ||
| ECC (testis epithelial) | GCT27D.C1 | |||
Average of cellular ATP levels of three independent assays were quantified using the Promega CellTiter-Glo Luminescent Cell Viability Assay kit.
The fraction of live cells was determined by FACS of nonpermeabilized cells stained with annexin-V and PI.
The fraction of cells with less <2N DNA was determined by FACS of permeabilized cells stained with PI. H1 ESCs and SCUi24 iPSCs differentiated spontaneously when cultured in standard conditions for fibroblast and epithelial cell lines (Fang et al., 2005). Teratocarcinoma xenografts were produced from ECCs inoculated subcutaneously into immunocompromised mice and treated by intraperitoneal injections of WX8. IC50 values for Apilimod (Figure S4) are indicated for NTERA-2 and HFF-1 cells as WX8/Apilimod.