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. 2021 Dec 11;23(3):447–458. doi: 10.1111/mpp.13171

FIGURE 2.

FIGURE 2

RSV infection has no effect on the splicing of NbbZIP60 mRNA. (a) Sanger sequencing results of NbbZIP60 cDNA in RSV‐infected or mock‐infected Nicotiana benthamiana plants. The total RNA of RSV‐infected or mock control plants was extracted at 14 days postinoculation (dpi) or 28 dpi for reverse transcription (RT)‐PCR and sequencing. (b) The splicing assays of NbbZIP60 in RSV‐infected N. benthamiana plants. The RT‐PCR products in (a) were subjected to flanking assays. The bands of NbbZIP60(u) and undigested products are labelled on the right; the DNA sizes are indicated on the left