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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: J Neurochem. 2021 Dec 29;160(4):469–481. doi: 10.1111/jnc.15564

Figure 3.

Figure 3

Prolonged ethanol exposure shifted constitutive 5-HT1AR internalization from a clathrin-dependent to a caveolin-dependent pathway. The constitutive 5-HT1AR internalization in 30 mM ethanol-treated cells (N=100 cells) was greater when compared to the vehicle treatment (N=120 cells). Pretreatment with concanavalin A (ConA, 250 μg/ml, 1hr at 37°C) blocked receptor internalization in Veh-treated cells (N=61 cells) but not in 30 mM ethanol-treated cells (N=54 cells). Nystatin treatment (25 μg/ml, 30 min at 37°C) diminished receptor internalization in 30 mM ethanol-treated cells (N=65 cells) and had no effect in Veh-treated cells (N=54 cells). Dynasore treatment (20 μM, 15 min at 37°C) abolished constitutive internalization in both Veh- (N=65 cells) and 30 mM ethanol-treated cells (N=52 cells). ****p<0.0001 vs. Veh