Table 1.
The twenty most enriched RNA molecules in the Lp-EVs as determined by RNAseq analyses.
| Label/DNA regiona | Annotationa | log2FCb | norm Lp-EV readsc | Similarityd |
|---|---|---|---|---|
| lpp0294 (f): 328282-328335 | UTR/start | 13 | 13 184 | hsa-miR 329-3p |
| lppnc0374 | sRNA (anti lpp1558) | 12 | 77 201 | - |
| lppt29 | tRNA-Phe | 12 | 304 066 | hsa-miR 5001-3p + 4451 |
| lppt25 | tRNA-Pro | 12 | 280 164 | - |
| lppt08 | tRNA-Pro | 10 | 29 498 | hsa-miR 6813-5p + 152-5p |
| lppt37 | tRNA-Met | 9 | 33 524 | - |
| lpp0956 (f): 1063203-1063309 | UTR | 9 | 6 110 | hsa-miR 4775 |
| lppnc0001 | sRNA RsmY | 9 | 349 673 | hsa-miR 144-3p |
| lppt04 | tRNA-Tyr | 9 | 81 954 | hsa-miR 4687-3p + 6819-5p |
| lppt39 | tRNA-Val | 8 | 104 797 | hsa-miR 323a-5p + 619-3p |
| lppt38 | tRNA-Pro | 8 | 44 788 | hsa-miR 6816-3p |
| lppt12 | tRNA-Leu | 8 | 26 718 | - |
| lppnc0692 | sRNA (intergenic lpp2966/67) | 8 | 25 277 | hsa-miR 6807-5p + 4773 |
| lppt34 | tRNA-Leu | 8 | 327 716 | - |
| lppt43 | tRNA-Leu | 7 | 134 92 | hsa-miR 6742-3p |
| lpp5011 (f): 2105717-2105648 | CDS | 7 | 17 070 | hsa-miR 3619-5p |
| lpp5013 (f): 2578647-2578735 | CDS | 7 | 9 863 | hsa-miR 4637 + 6751-3p |
| lppt06 | tRNA-Thr | 6 | 648 296 | hsa-miR 302b-5p + 6077 |
| lppnc0047 | sRNA | 6 | 6 946 | hsa-miR 1290 + 329-5p |
| lppt41 | tRNA-Met | 6 | 845 116 | hsa-miR 769-5p |
aLabel-DNA region; sRNA label as defined in Sahr et al., 2017.
bLog2FC, Enrichment of RNA molecules in the Lp-EVs as compared to the bacterial culture defined as log 2 fold change (FC).
cnorm Lp-EV reads, Number of reads detected for each sRNA within the Lp-EVs after normalization.
dSimilarity, similarity found to a human sapiens micro RNAs (has-miR), 5p, 5′ end, 3p, 3′end, inverted, similarity with the inverted sequence; CDS coding sequence.