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. 2022 Feb 9;13:759. doi: 10.1038/s41467-022-28407-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Enrichment plots (GSEA) and heatmap of genes related to the positive regulation of phagocytosis in CD11b⁺ cells from 7-day WGP-trained as compared to PBS mice. b The percent of CD45⁺ pancreatic cells that phagocytosed a pHrodo Green Staph Aureus particle in PBS (n = 10) and 7-day WGP (n = 9) mice along with the MFI of the pHrodo Green Staph Aureus particle. Each dot represents data from one mouse. *p = 0.043, ****p < 0.0001. c The percent of CD11b + myeloid pancreatic cells that phagocytosed a pHrodo Green Staph Aureus particle in PBS (n = 13) and 7-day WGP (n = 13) mice. Cells were first gated on the CD45⁺ population. The MFI of the pHrodo Green Staph Aureus particle is shown. **p = 0.0021, ***p = 0.0002. d The percent of CD11b⁺ myeloid pancreatic cells that phagocytosed KPC^GFP+ tumor cells in PBS (n = 5) and 7-day WGP (n = 5) mice. *p = 0.0103. Cells were first gated on the CD45⁺ population. The summarized MFI of the GFP⁺ signal is also shown. *p = 0.0379. e Enrichment plots (GSEA) and heatmap of genes related to the reactive oxygen species biosynthetic processes and the positive regulation of reactive oxygen species metabolic processes in CD11b⁺ cells from 7-day WGP-trained as compared to PBS mice. f Summarized results from a cytotoxicity assay where CD11b⁺ cells from PBS and 7-day WGP-trained mice were sorted and incubated at a ratio of 1:20 KPC^Luc+: CD11b⁺ cells for 24 h. NAC was used to block ROS expression and the tumor cytotoxicity was assessed by luminescence (PBS n = 3, WGP n = 4). g Summarized data from cytotoxicity assays where CD11b⁺ cells from 7-day WGP-trained mice were sorted and incubated at a ratio of 1:20 KPC^Luc+: CD11b⁺ cells for 24 h. Trolox or DFO or respective vehicle controls were added and tumor cytotoxicity was determined by measuring luminescence (n = 3). *p = 0.0255, **p = 0.0019. An unpaired, two-tailed student’s t-test was used in b–d and g and a one-way ANOVA with multiple comparisons was used for f. Data were represented as mean ± SEM. ns not significant. In b–g, each sample represents a biologically independent animal obtained over a single independent experiment which was repeated at least twice for verification of results.