Figure 1.

LysoTracker measurements of fibroblasts from individuals with NPC1 are reproducible. Primary fibroblast cell lines established from 24 individuals with NPC1 were previously stained with LysoTracker, and these LysoTracker levels were measured by FACS for each cell line⁴². Several years later, fibroblasts from these 24 cell lines were again subjected to the same LysoTracker staining protocol. Measurements from both studies were paired for each patient, and correlation analysis showed significant correlation of the paired measurements for these NPC1 cell lines (Spearman r = 0.7652, p < 0.0001). Line was calculated using simple linear regression.