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. 2022 Feb 9;13:765. doi: 10.1038/s41467-022-28350-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Strain Engineering Repositories contain all the digital footprint produced during the engineering of a cell line. The repository provides general information about the cell line project (in this example, the barcoding proof of principle of S. cerevisiae). It also contains all the different “commits” that were made during the engineering process. b A commit represents a related set of changes introduced into a cell line. All commits have a unique digital identifier and some commits (decided by the user) may also have a physical identifier barcode that is physically inserted into the cell chromosome. Recovering the barcode by sequencing allows a cell engineer to recover the id of the commit containing all the digital footprint of the cell line.