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. 2022 Feb 9;13:757. doi: 10.1038/s41467-022-28416-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The scheme of CICI. LacO and TetO arrays were inserted into two genomic loci. Fusion proteins LacI-FKBP12 and TetR-FRB associated with these arrays dimerize in the presence of rapamycin. LacI-GFP and TetR-mCherry were also included for visualization. B LacO (green dot) and TetO (red dot) arrays are integrated 150 kb from the centromere on the left and right arms of Chr XV. C Visualization of the CICI strain (containing the complete CICI system in (A)) and the control strain (containing LacI and TetR instead of LacI-FKBP12 and TetR-FRB) ± rapamycin. D Histograms of distances between the two dots in the CICI strain (upper panel) and in the control strain (lower panel) ± rapamycin. The +rapamycin histogram includes measurements where the CICI or control strains were incubated with rapamycin for 1–2 h. In total, 116, 102, 407, and 308 cells were measured, respectively. The co-localization fraction in each condition is shown in the plots. E Fraction of co-localization as a function of rapamycin incubation time in the CICI (red) or the control strain (black). The total numbers of data points were 436 for CICI and 912 for control. Data are presented as mean values ± SEM. Source data for D and E are provided as a Source Data file.