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. 2022 Feb 9;13:757. doi: 10.1038/s41467-022-28416-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Example time-lapse images of CICI strain (pair 5). The chromatin dots at the LacO and TetO arrays are circled. The budneck is labeled with Myo1-mCherry. B Distance between LacO and TetO arrays as a function of time in three cells. The upper panel is derived from the mother cell in (A). The horizontal line represents the co-localization threshold (0.4 µm), and the vertical lines show the time of cell division. The gray arrows point to the CICI formation time in these three single cells. C Histogram of CICI formation time for all cells (LacO and TetO pair 5, N = 773). The histogram is fitted with double exponential curves, and the time constants and amplitudes of the two phases (t₁, t₂, A₁, and A₂) are shown. D Histogram of CICI formation time for cells divided into two different cell cycle stages (N = 480 and 255 for G1 and budded cells, respectively). The double exponential fit has the same t₁ and t₂, but different A₁ and A₂. E, F Same as C and D, except that LacO and TetO arrays are placed on the two sides of the nucleolus (pair 4). N = 378, 220, 135 for all cells, G1 cells and budded cells, respectively. Source data for B–F are provided as a Source Data file.