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. 2022 Feb 9;13:757. doi: 10.1038/s41467-022-28416-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Example time-lapse images of CICI strain (pair 5) that show CICI disruption near the end of the cell cycle. B Distance between LacO and TetO arrays as a function of time in two cells. The upper panel is derived from the mother cell in (A). The horizontal line represents the co-localization threshold (0.4 µm), and the vertical lines show the time of cell division. The gray arrows point to the time of CICI disruption. C Histogram of the time of CICI disruption relative to the end of the cell cycle (total CICI disruption events N = 36). The seven disruption events near the end of the cell cycle are statistically significant (one-sided Student’s t-test, P value < 0.001). D Model of CICI disruption during anaphase. For clarity, only one of the two CICI pairs is shown in these cells. Source data for B and C are provided as a Source Data file.