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. 2022 Feb 9;13:757. doi: 10.1038/s41467-022-28416-3

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Engineered proximity between MATa and HMRα-B by CICI. Green and Red dots: LacO and TetO arrays; Flash symbol: HO digestion. B Visualization of the MATa and HMRα-B loci ± rapamycin. C Fraction of co-localization as a function of rapamycin incubation time in the CICI (red) or the control strain (black) used in the mating-type switching assay. The total numbers of data points were 226 for the CICI strain and 272 for the control. Error bars represent standard error in the fraction. D Quantitative analysis of HMR usage in yeast mating-type switching. Top gel: PCR of the MATa sequence, which decreases after the HO induction (representing the switching from a to α or α-B). Bottom gel: PCR of the MATα sequence followed by BamHI digestion. Undigested (top arrow)/digested bands (bottom two arrows) represent the usage of HML (α) and HMR (α-B). 1 kb DNA ladder is shown on the right. E The fraction of HMR usage for the four conditions in D, which was calculated as the sum intensity of the lower two bands divided by the sum intensity of all three bands. Data are presented as mean values ± SEM from three biological repeats. Source data for C–E are provided as a Source Data file.