Table 6.

Analysis of variance (ANOVA) of response surface quadratic model for the production of l-asparaginase

Source	Sum of squares	df	Mean square	F value	p value prob > f
Model	2918.75	14	208.48	165.72	< 0.0001*	Significant
A—l-asparagine	1283.65	1	1283.65	1020.3	< 0.0001*
B—Peptone	79.12	1	79.12	62.89	< 0.0001*
C—Glucose	186.15	1	186.15	147.97	< 0.0001*
D—Na₂HPO₄	290.56	1	290.56	230.96	< 0.0001*
AB	27.58	1	27.58	21.92	0.0003*
AC	1.37	1	1.37	1.09	0.3127
AD	1.884E−0.003	1	1.884E−0.003	1.497E−0.003	0.9696
BC	37.1	1	3.17	2.52	0.1335
BD	0.68	1	0.68	0.54	0.4735
CD	14.92	1	14.92	11.86	0.0036*
A2	343.56	1	343.56	273.09	< 0.0001*
B2	352.03	1	352.03	279.83	< 0.0001*
C2	461.83	1	461.83	367.1	< 0.0001*
D2	310.68	1	310.68	246.95	< 0.0001*
Residual	18.87	15	1.26
Lack of fit	10.81	10	1.08	0.67	0.7239	Not significant
Pure error	8.06	5	1.61
Cor total	2937.63	29

*Statistically significant at 95% confidence level (p value < 0.05)