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. 2022 Feb 9;12:2210. doi: 10.1038/s41598-022-05749-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative micrographs of pulmonary adenocarcinoma and its in-situ PCR detection of mycobacterial DNA. Left figure shows well differentiated adenocarcinoma stained with haematoxylin/eosin, neoplastic cells show big hyperchromatic basophilic nucleus. The right figure shows high power magnification of neoplastic gland, some cells show positivity for mycobacterial DNA (IS-6110) detected by in situ RT-PCR (dark blue dots, arrows) in the nucleus stained by nuclear fast red.