Fig. 4.

Neutralization of SARS-CoV-2 VOC and VOI by monomeric and bivalent Nbs. a Neutralization of pseudotyped SARS-CoV-2 variants by Nb1, Nb2, or Nb1–Nb2, respectively. Pseudovirus was pre-incubated with tenfold serially diluted Nbs before inoculation of Huh7 cells. At 48 h post infection, luciferase activities were measured, and percent neutralization was calculated. The experiments were performed independently at least twice, and similar results were obtained. One representative data of one experiment are shown and data were average values of three replicates (n = 3). b Determination of neutralization efficacy of bivalent Nb1–Nb2 against recombinant SARS-CoV-2 GFP/ΔN trVLP. The infected cells were subjected to flow cytometry analysis for quantifying the GFP fluorescence at 2 days post infection. Error bars represent the standard deviations from three independent experiments (n = 3)