Table 3.
Weight average (Mw) and number average (Mn) molecular weights, and polydispersity (P), as calculated from UV-detected HPSEC chromatograms for HA-LIG and HA-CYN, before and after addition of acetic acid (AcOH). Standard deviation was < 5%.
| Sample | Peak interval (mL) | Mw | Mn | P |
|---|---|---|---|---|
| HA LIG | 7.5–18.0 | 11,957 | 1774 | 6.7 |
| HA LIG + AcOH-peak 1 | 7.5–9.2 | 21,792 | 21,314 | 1.0 |
| HA LIG + AcOH-peak 2 | 9.2–10.5 | 10,374 | 10,172 | 1.0 |
| HA LIG + AcOH-peak 3 | 10.5–14.9 | 4238 | 3396 | 1.2 |
| HA LIG + AcOH-peak 4 | 15.2–22.3 | 180 | 87 | 2.1 |
| HA CYN-peak 1 | 4.7–6.3 | 86,258 | 85,187 | 1.0 |
| HA CYN-peak 2 | 6.3–8.7 | 41,437 | 38,391 | 1.1 |
| HA CYN-peak 3 | 8.7–9.3 | 18,489 | 18,412 | 1.0 |
| HA CYN + AcOH-peak 1 | 4.7–6.3 | 84,536 | 83,560 | 1.0 |
| HA CYN + AcOH-peak 2 | 6.3–8.7 | 37,934 | 34,658 | 1.1 |
| HA CYN + AcOH-peak 3 | 9.2–9.6 | 13,783 | 13,724 | 1.0 |