Fig. 8.

Ddr2 deficiency inhibits chondrogenic differentiation in micromass cultures. a Chondroprogenitor cells isolated from limb buds of E12.5 Ddr2^fl/fl mice were treated with AdLacz or AdCre and differentiated for 8 days followed by alcian blue staining (top two panels) or were subsequently stained with eosin (bottom panel). Scale bar: 500 μm. b Total DNA. b, c–f Quantitative real-time RT-PCR (qRT-PCR) gene expression analyses of micromass cultures. b, c Efficient knockdown of Ddr2. c Gene expression analysis of chondrocyte differentiation markers. d–g Gene expression analysis shows downregulation of chondrocyte and hedgehog signaling in Ddr2-deficient chondrocytes. Unpaired t-test *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.000 1; ns Not significant