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. 2022 Feb 9;13:766. doi: 10.1038/s41467-022-28341-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Of 109 studied TFs, 80 had 202 interactions with other studied TFs. Blue edges indicate interactions from the BioID analysis, red from the AP-MS analysis and green from both. b Most of these TF-TF (118) interactions were TF interactions with NFIs (left panel). The right panel shows the separate groups shared by one or multiple NFIs. Color code: Green nodes = NFIs, yellow nodes = interactions to NFIs, orange nodes = interactions from NFIs, red nodes = interactions to and from NFIs and gray nodes = no interactions to or from NFIs. Color coding of the nodes is shown on the right side of the figure. The edge weight displays the average spectral count value detected for the corresponding interaction. c NFIA was silenced using siRNA transfection, and NFIA levels were detected 48 h after transfection by western blotting using a specific antibody against NFIA. d TF activity was investigated after NFIA silencing using both repressive and activating reporter gene analysis. Both the repressing and activating functions of KLF4 were reduced upon NFIA silencing. In addition, SOX2 and PAX6 activity was reduced, while HME1 activity was increased upon NFIA silencing. The representative data from two repeated experiments are presented as mean values +SD as appropriate. Two-sided t-test was used. N = 3, ***p < 0.001, **p < 0.01, *p < 0.05. Source data of the figure are provided as a Source Data file.