Figure 4.

Growth of Pseudomonas aeruginosa PAO1 ΔwspF P_BADalg strains reveals mutation of R249 and Y256 impacts cell viability during alginate production. After reaching an OD₆₀₀ of approximately 0.5, cells were induced to produce alginate with 0.5% (w/v) L-arabinose. Data points represent averages from three biological replicates with two technical replicates per biological replicate. A, control strains. B, ΔalgL strains complemented with AlgL point mutants that retained alginate lyase enzymatic activity in vitro. C, ΔalgL strains complemented with AlgL point mutants whose alginate lyase enzymatic activity in vitro was compromised.