Table 2.
Proposed role of P. aeruginosa AlgL residues and the point mutants used in this study
| Residue | Proposed function | Point mutants studied |
|---|---|---|
| K66 | Lid-loop residue directly involved in substrate binding | K66A |
| H202 | Neutralizes anionic intermediate of the substrate | H202A |
| W205 | Highly conserved residue indirectly involved in substrate binding | W205F |
| R249 | Neutralizes C-5 carboxylate group on substrate for proton abstraction | R249A, R249E, R249K |
| Y256 | Catalytic acid/base | Y256F |
| Y259 | Directly involved in substrate binding | Y259F |