Table 3.
Pseudomonas aeruginosa AlgL reaction steady-state kinetic parametersa
| AlgL enzymea | kcat/KM (s−1 M−1) | kcat (s−1) | KM (μM) |
|---|---|---|---|
| WT | 147 ± 10.6 × 103 | 15.7 ± 0.274 | 107 ± 5.80 |
| K66A | 1.37 ± 0.141 × 103 | 0.128 ± 0.00303 | 93.5 ± 7.44 |
| H202A | Activity not detected | ||
| W205F | 3.89 ± 0.350 × 103 | 1.92 ± 0.0647 | 494 ± 27.9 |
| R249K | 2.23 ± 0.429 × 103 | 0.322 ± 0.0165 | 144 ± 20.3 |
| R249A | Activity not detected | ||
| R249E | Activity not detected | ||
| Y256F | Activity not detected | ||
| Y259F | 44.5 ± 7.05 × 103 | 15.8 ± 0.864 | 354 ± 36.8 |
a
Initial velocities were fitted to the Michaelis–Menten equation.