Figure 7.

Hypermethylation of miR-145 promoter suppresses miR-145-5p expression. a, miR-145-5p expression in CD and HC (CD, n = 10; HC, n = 10). b-i, Methylation analysis of total methylation ratio and relative methylation ratio of each CpG site. j, FISH of miR-145-5p (green stain), and IF of SOX9 (red stain) and CLDN8 (pink stain) in the colonic mucosa of patients with CD and HCs. Scale bars, 100 μm. All Data are shown as means ± SEM in (a), (b), (d), (e), (f), (g), (h) and (i). Student's t-test were used for com-parison between two groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 as compared with the control.