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. 2022 Feb 4;76:103846. doi: 10.1016/j.ebiom.2022.103846

Figure 8.

Figure 8

Hypermethylation of miR-145 promoter-mediated SOX9-CLDN8 pathway. a-d, The expression of miR-145-5p, SOX9 and CLDN8 in NCM460 cells treated with TNF-a (50 ng/ml) or 5-aza (5 μmol/L). e-l, Methylation analysis of total methylation ratio and relative methylation ratio of each CpG site of miR-145 promoter in NCM460 cells treated with TNF-a (50 ng/ml) or 5-aza (5 μmol/L). m-n, TEER and FD4 permeability of the Caco-2 cell monolayer. o, The proposed model of the miR-145-5p/SOX9/CLDN8 pathway. Aberrant hypermethylation of miR-145-5p promoter mediated activation of SOX9 expression to negatively regulate CLDN8, which aggravates the intestinal epithelial TJ barrier dysfunction. All Data are shown as means ± SEM in (a), (b), (c) and (d). Student's t-test and one-way ANOVA were used for comparison, respectively. ns: no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 as compared with the control.