FIG. 1.

(A) Strategy of construction of recombinant plasmid pBgDdTM. PCR was performed using pBlueSK+2.6 (1), which contains the gD gene of B virus, as the template together with primers S and R. The PCR product (shaded column) was replaced with a KpnI-KpnI fragment of pBgD. (B) Schematic representation of gD and gDdTM of B virus. The numbers indicate amino acid residues. The shaded columns represent the signal peptide (SP), TM, and CT. Positions of potential N-glycosylation sites (bar) and cysteine residues (inverted triangles) are illustrated on each of the columns.