FIG. 3.

Reactivity of recombinant gD to monkey sera in RIPA. Radiolabeled extracts from COS7 cells transfected with the pBgD (D) or pcDNA vector DNA (V) were immunoprecipitated with various sera (dilution, 1:50). Sera from monkeys regarded as B virus-infected (no. 37, 41, 42, 46, 49, 82, 83, and 84) and noninfected (no. 991,992, 993, 994, and 995) were used. Anti-BV serum was also included, and the position of the gD was indicated on the left of the panel. Molecular mass standards (in kilodaltons) are shown on the right.